Enzymic Detection of Adhesion of EnteropathogenicEscherichia colito HEp-2 Cells
نویسندگان
چکیده
منابع مشابه
M protein mediates streptococcal adhesion to HEp-2 cells.
Streptococcus pyogenes adheres to human epithelial cells in vitro and in vivo. To identify adhesins, cell wall components were extracted from S. pyogenes M6 with alkali or by treatment with mutanolysin and lysozyme. HEp-2 cells were incubated with extracts of S. pyogenes M6 and then analyzed by Western blot (immunoblot) assays, using antibodies to S. pyogenes. Only one streptococcal component (...
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Plasmid-encoded adherence factors have been shown to be important for the full expression of enteropathogenic Escherichia coli (EPEC) pathogenicity and for EPEC adhesion to cultured HEp-2 cells. EPEC strain E2348 (O127) shows localized HEp-2 cell adhesion and possesses a 60-megadalton plasmid, pMAR2. When E2348 is cured of pMAR2 it loses the ability to adhere to HEp-2 cells, while nonadherent E...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1987
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1987.tb03146.x